ZC Bioscience
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Recombinase-Aided Amplification

Isothermal nucleic acid detection.
Results in 5–15 minutes.

Runs at 37–42 ℃ with no thermal cycler. Lyophilised, ready-to-use reagents. Built for the field.

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8-tube strip of lyophilised RAA reagents

Lyophilised RAA reagent strip. All reagents are for research use only (RUO).

Technology

RAA. Amplification without the heat cycle.

One temperature. One primer pair. One tube.

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DNA double helix render

Recombinase-Aided Amplification (RAA) is a rapid isothermal nucleic acid amplification technology. A recombinase obtained from bacteria or fungi binds tightly to the primer DNA at ambient temperature, forming a recombinase–primer complex. When the primer locates its fully complementary sequence on the template, single-stranded DNA-binding protein helps open the double strand, and DNA polymerase synthesises a new complementary strand. The product accumulates exponentially.

With a labelled fluorescent probe the reaction can be read in real time; a fluorescence result is typically available within 5–15 minutes. All reaction components are pre-mixed and lyophilised in the tube — add Buffer A, Buffer B and the extracted sample DNA, then read the curve.

RAA can in principle be multiplexed: two or more primer pairs in one reaction amplify several targets simultaneously, saving reagents and cost. It is applied across food safety (pathogen detection, meat-species authentication, GMO screening), aquaculture pathogen detection, animal disease detection and other fields.

  • Simple operation

    Two-step workflow. Molecular-level testing that does not require specialist staff.

  • Fast

    Results in 5–15 minutes; truly rapid testing.

  • Accurate

    High sensitivity and specificity; precise recognition of the target sequence.

  • Portable

    Compact instruments suited to harsh field conditions and on-site inspection.

  • Isothermal

    Runs at 30–42 ℃; no thermal cycler or other expensive equipment required.

  • Lyophilised

    Freeze-dried pellets, ready to use, easy to ship, and free from freeze–thaw loss.

Compare

Which platform is right for your workflow?

RAA next to conventional PCR and LAMP.

RAAPCRLAMP
EnzymesRecombinase, SSB, strand-displacing DNA polymeraseTaq DNA polymeraseStrand-displacing DNA polymerase
Temperature37–39 ℃, isothermalHigh, thermal cycling60–65 ℃, isothermal
Time to result15 min1.5 h40 min
Primer pairs112
Reagent formatLyophilised pelletLiquidLiquid
ReadoutGel; real-time fluorescence; lateral-flow stripGel; real-time fluorescenceVisual turbidity
EquipmentHeat block, water bath or portable fluorescence analyzerThermal cycler / qPCR instrumentHeat block or turbidimeter
Limitations noted—SlowAerosol carry-over, false positives

Values as published on the ZC Bioscience platform comparison. Actual run times depend on assay and instrument.

Products

RAA Core Amplification Kits

Six enzyme reagent kits. DNA or RNA. Gel, fluorescence or strip readout.

Request a Quote How RAA works
Gloved hand holding an 8-tube strip of lyophilised RAA reagent pellets

Spec sheet

48 tests/kit · 50 μL reaction · lyophilised · store at −20 ℃, dry and dark · shelf life 12 months · primers 30–35 bp, probes 46–52 bp. Rapid: 30 min at 30–42 ℃ (optimum 37–39 ℃) yields a gel-detectable product. Sensitive: over 10⁷-fold amplification of the target.

S001ZCRAA Basic Nucleic Acid Amplification Kit (DNA, gel readout) (RUO)37 ℃ · agarose gel · heat block / water bathRequest a Quote
S002ZCRAA Fluorescent Nucleic Acid Amplification Kit (DNA, real-time) (RUO)Real-time on Genchek or qPCR instruments · 5–20 minRequest a Quote
S003ZCRT-RAA Basic Nucleic Acid Amplification Kit (RNA, gel readout) (RUO)37 ℃ · reverse transcription + gel readoutRequest a Quote
S004ZCRT-RAA Fluorescent Nucleic Acid Amplification Kit (RNA, real-time) (RUO)Real-time · 5–20 minRequest a Quote
S005ZCRAA-nfo Nucleic Acid Amplification Kit (Lateral-Flow Strip, DNA) (RUO)39 ℃ isothermal · universal lateral-flow stripRequest a Quote
S006ZCRT-RAA-nfo Nucleic Acid Amplification Kit (Lateral-Flow Strip, RNA) (RUO)42 ℃ isothermal · universal lateral-flow stripRequest a Quote

Instruments

Genchek Isothermal Fluorescence Analyzer

Eight wells. Two channels. Built-in reagent mixing. Fits in a field case.

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Genchek isothermal fluorescence analyzer, portable 8-well unit with touchscreen

An integrated, portable rapid-test analyzer for on-site and mobile testing. All assays run under one software interface and results are read from a single display. Real-time fluorescence curves, automatic data analysis and report generation with multiple export routes.

Throughput
8 wells × 2 channels (up to 16 targets/run)
Fluorescence channels
FAM / ROX
Temperature range
Ambient to 65 ℃
Temperature accuracy
±0.5 ℃
Net weight
3.8 kg
Dimensions (L×W×H)
255 × 240 × 123 mm
Power
120 W · AC 100–240 V, 50/60 Hz
Operating environment
5–35 ℃, 20–80 % RH
Operating system
OpenRTOS

Compatible reagents also run on standard real-time PCR instruments (ABI, MJ, Roche, Bioer series).

Sample preparation

Rapid Nucleic Acid Extraction Kits

RAA-ready DNA or RNA in 5 to 25 minutes. No hazardous solvents.

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Gloved technician transferring sample in a clean bench

Spec sheet

Store at room temperature, dry · shelf life 12 months · purpose-built to yield RAA-ready template · low cross-contamination risk.

ND01ZCRapid DNA Extraction Reagent, heat-lysis (RUO)50 preps · 5 min · animal tissue, blood, meat, plant tissue, seeds, bacteriaRequest a Quote
ND02ZCUniversal Rapid DNA Extraction Kit, spin column (RUO)50 preps · 15 min · lyse–bind–wash–eluteRequest a Quote
ND03ZCUniversal Rapid RNA Extraction Kit, spin column (RUO)50 preps · 10 min · tissue, cells, saliva, blood, swabs, faecesRequest a Quote
ND06ZCMagnetic-Bead DNA Extraction Pre-filled Plate, for automated extractors (RUO)96 preps · 25 min · nano-magnetic-bead purification, high throughputRequest a Quote

Food safety

Food Safety Molecular Detection Series

Seventeen RAA fluorescent kits across meat species and foodborne pathogens, plus GMO screening.

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Magnifying glass over a DNA strand

Common to the series

24 tests/kit · lyophilised · 30–42 ℃ (optimum 37–39 ℃) · result read directly from the amplification curve, no electrophoresis · store at −20 ℃, protected from light · shelf life 6 months · runs on Genchek-I/II or standard real-time PCR instruments.

Animal species (meat authentication) — 10 kits

Species-specific gene fragment; limit of detection 1 % (w/w); no cross-reaction observed with common species. For animal tissue, blood, foods, meat products (incl. processed meat) and feed.

A101ZCChicken-Derived Ingredient Molecular Detection Kit (RAA, Fluorescent) (RUO)24 testsRequest a Quote
A102ZCDuck-Derived Ingredient Molecular Detection Kit (RAA, Fluorescent) (RUO)24 testsRequest a Quote
A103ZCPork-Derived Ingredient Molecular Detection Kit (RAA, Fluorescent) (RUO)24 testsRequest a Quote
A104ZCBovine-Derived Ingredient Molecular Detection Kit (RAA, Fluorescent) (RUO)24 testsRequest a Quote
A105ZCSheep & Goat-Derived Ingredient Molecular Detection Kit (RAA, Fluorescent) (RUO)24 testsRequest a Quote
A106ZCRat-Derived Ingredient Molecular Detection Kit (RAA, Fluorescent) (RUO)24 tests · fresh and processed meatRequest a Quote
A107ZCHorse-Derived Ingredient Molecular Detection Kit (RAA, Fluorescent) (RUO)24 testsRequest a Quote
A108ZCMink-Derived Ingredient Molecular Detection Kit (RAA, Fluorescent) (RUO)24 tests · fresh and processed meatRequest a Quote
A109ZCFox-Derived Ingredient Molecular Detection Kit (RAA, Fluorescent) (RUO)24 tests · fresh and processed meatRequest a Quote
A110ZCDonkey-Derived Ingredient Molecular Detection Kit (RAA, Fluorescent) (RUO)24 testsRequest a Quote

Foodborne pathogens — 7 kits

Qualitative detection; result in 5–15 min; limit of detection 10³ cfu/mL.

B201ZCStaphylococcus aureus Molecular Detection Kit (RAA, Fluorescent) (RUO)Foods, cosmetics, disposable hygiene productsRequest a Quote
B202ZCSalmonella Molecular Detection Kit (RAA, Fluorescent) (RUO)FoodsRequest a Quote
B203ZCPseudomonas aeruginosa Molecular Detection Kit (RAA, Fluorescent) (RUO)Water and beveragesRequest a Quote
B204ZCListeria monocytogenes Molecular Detection Kit (RAA, Fluorescent) (RUO)FoodsRequest a Quote
B205ZCHaemolytic Streptococcus Molecular Detection Kit (RAA, Fluorescent) (RUO)Foods, cosmetics, disposable hygiene productsRequest a Quote
B206ZCVibrio parahaemolyticus Molecular Detection Kit (RAA, Fluorescent) (RUO)Fish, shrimp, crab, shellfish, seaweedRequest a Quote
B207ZCShigella Molecular Detection Kit (RAA, Fluorescent) (RUO)FoodsRequest a Quote

GMO screening — catalogue listing

RAA fluorescent kits (RUO) for six regulatory elements and six events, 24 tests/kit. Catalogue numbers on request.

hpt geneBt genenos terminatorCaMV 35S promoterFMV 35S promoterbar gene Soybean GTS 40-3-2Soybean MON89788Maize MON810Maize Bt11Canola T45Canola Oxy-235

Instruments

Genchek Fully Automated Isothermal Nucleic Acid Detection System

Sample in, result out. Thirty minutes, hands-off.

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Performs quantitative pipetting, sample lysis, nucleic acid purification, isothermal amplification, fluorescence detection, analysis and reporting in one instrument. Load the sample and the system does the rest.

Run time
~30 min
Sample throughput
1
Fluorescence channels
FAM / ROX
Interface
7-inch colour touchscreen
Weight
16 kg
Dimensions (L×W×H)
260 × 325 × 420 mm
Power
221 W · AC 220 V, 50 Hz
UV lamp / air purification
Yes / Yes
Operating environment
20–30 ℃, 20–80 % RH

Pipetting: <15 μL accuracy >98 %, CV <5 %; 15–50 μL accuracy >98.5 %, CV <3 %; >50 μL accuracy >99 %, CV <1.2 %. Product photography available on request.

Solutions

Three questions. One workflow.

What species is in the meat? Is a pathogen present? Is it genetically modified? Same instrument, same 5–15 minute readout.

DNA helix with two researchers

Meat species authentication

As price gaps between meats widen, species substitution and adulteration are increasingly common. RAA-based species kits detect species-specific genes, identifying meat origin at the molecular level with no cross-reaction between species. Limit of detection 1 % adulterated meat; lyophilised format; 5–15 min.

10 targetsChicken · Pork · Duck · Beef · Sheep/Goat · Rat · Horse · Mink · Fox · Donkey
UsersFood and feed manufacturers, dietary-supplement producers, customs and border inspection, livestock authorities, agricultural research institutes, third-party testing laboratories.
See the kits

Foodborne pathogen detection

In China, pathogenic bacteria account for an estimated 40–50 % of reported foodborne-illness cases each year. RAA pathogen kits combine multi-channel detection, short run times and high sensitivity (10³ cfu/mL) — raising testing throughput and moving food-safety warnings earlier. Qualitative detection in food, beverages, cosmetics and seafood.

9 targetsStaphylococcus aureus · Salmonella · Pseudomonas aeruginosa · Listeria monocytogenes · Haemolytic Streptococcus · Vibrio parahaemolyticus · Shigella · E. coli O157:H7 · Vibrio cholerae
Method referenceGB 4789 series and SN/T methods (e.g. GB 4789.4, GB 4789.10, GB 4789.30).
See the kits

GMO screening

GM crops carry genes introduced from other organisms to alter traits, nutrition or quality, and regulators worldwide require labelling and control. RAA-based GMO kits specifically detect exogenous elements and events in crops, identifying GM material at the molecular level.

6 screening elementsBt · bar · hpt · CaMV 35S promoter · FMV 35S promoter · nos terminator
6 eventsSoybean GTS 40-3-2, MON89788 · Maize MON810, Bt11 · Canola T45, Oxy-235
See the kits

Example workflow (animal-species assay): weigh 50 mg sample into the extraction solution, incubate at 70 ℃ for 10 min; add 45.5 μL Buffer A, 2.5 μL Buffer B and 2 μL sample nucleic acid to the reagent tube; place in the Genchek analyzer; result in 5–15 min.

Services

From first question to custom target.

Technical support, after-sales service and custom RAA assay development.

Technician working at a laboratory instrument

Technical support

Our R&D team answers every product question with patient explanation and demonstration. We provide consultation, technical documentation, training and after-sales support.

Contact support
Technician pipetting inside a clean bench

After-sales service

Good after-sales service earns customer trust. We provide long-term technical documentation and support; our engineers respond promptly and resolve issues remotely by email or phone, Mon–Fri 9:00–18:00 (UTC+8).

+86 571 8861 9668
Molecular laboratory with instruments and workstations

Custom assay development

RAA is a versatile, portable rapid-testing platform that can replace LAMP or PCR in many workflows. We develop custom RAA assays for new targets and support OEM supply — contact us to discuss your target.

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Company

Hangzhou ZC Bio-Sci & Tech Co., Ltd.

An R&D-driven biotechnology company in Hangzhou, China.

ZC Bioscience develops and applies Recombinase-Aided Amplification (RAA), an isothermal nucleic acid amplification technology, and supplies RAA-based reagents, extraction kits and portable fluorescence instruments for rapid molecular testing. Since September 2016 the company has accelerated product development for RAA-based rapid food-safety testing, launching in turn the animal-species, foodborne-pathogen and GMO molecular detection series. Other application areas include environmental monitoring, aquaculture pathogen detection and animal disease detection.

ZC Bioscience has signed strategic cooperation agreements with government institutions and research organisations across several fields to co-develop RAA-based detection kits. We remain committed to advancing RAA technology and delivering rapid-testing products and services that are fast, accurate and easy to use. All reagents are for research use only.

  • 500 m²Laboratory space, plus 400 m² of offices
  • RMB 12M+Installed equipment value
  • 90 %Of the R&D team hold master's degrees or higher
  • 5050Hangzhou Binjiang District Talent Program; key enterprise cultivated by the district
Laboratory corridor with observation window Technician operating laboratory equipment Technician working inside a biosafety cabinet Clean molecular laboratory

News

Newsroom

Contact

Request a Quote.

Tell us your target and sample type. We reply within two business days.

AddressTianhe Hi-Tech Park, Bldg 5
688 Bin'an Rd, Binjiang District
Hangzhou 310052, China

Emailzcsw2017@163.com

Phone+86 571 8861 9668

HoursMon–Fri 9:00–18:00 (UTC+8)

Reagents are for research use only. Not for use in diagnostic procedures.